KMID : 0545120200300111614
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Journal of Microbiology and Biotechnology 2020 Volume.30 No. 11 p.1614 ~ p.1625
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The Anti-Inflammatory Effect of Trichilia martiana C. DC. in the Lipopolysaccharide-Stimulated Inflammatory Response in Macrophages and Airway Epithelial Cells and in LPS-Challenged Mice
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Park Ji-Won
Ryu Hyung-Won Ahn Hye-In Min Jae-Hong Kim Seong-Man Kim Min-Gu Kwon Ok-Kyoung Hwang Da-Seul Kim Soo-Yong Choi Sang-Ho Zamora Nelson Rosales Kattia Oh Sei-Ryang Lee Jae-Won Ahn Kyung-Seop
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Abstract
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A number of species of the genus Trichilia (Meliaceae) exhibit anti-inflammatory effects. However, the effect of Trichilia martiana C. DC. (TM) on lipopolysaccharide (LPS)-induced inflammation has not, to the best of our knowledge, yet been determined. Therefore, in the present study, the antiinflammatory effect of TM on LPS-stimulated RAW264.7 macrophages was evaluated. The ethanol extract of TM (TMEE) significantly inhibited LPS-induced nitric oxide (NO), prostaglandin 2 (PGE2), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). TMEE also reduced the levels of inflammatory cytokines, including tumor necrosis factor-alpha (TNF-¥á), interleukin (IL)-1¥â and IL- 6. The upregulation of mitogen-activated protein kinases (MAPKs) and NF-¥êB activation was revealed to be downregulated following TMEE pretreatment. Furthermore, TMEE was indicated to lead to the nucleus translocation of nuclear factor erythroid-derived 2-related factor 2 (Nrf2) and the expression of heme oxygenase-1 (HO-1). In H292 airway epithelial cells, the pretreatment of TMEE significantly downregulated the production of LPS-stimulated IL-1¥â, and TMEE was indicated to increase the expression of HO-1. In animal models exhibiting LPS-induced acute lung injury (ALI), treatment with TMEE reduced the levels of macrophages influx and TNF-¥á production in the bronchoalveolar lavage fluid (BALF) of ALI mice. Additionally, TMEE significantly downregulated the activation of ERK, JNK and I¥êB, and upregulated the expression of HO-1 in the lungs of ALI mice. In conclusion, the results of the current study demonstrated that TMEE could exert a regulatory role in the prevention or treatment of the endotoxin-mediated inflammatory response.
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KEYWORD
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Trichilia martiana C. DC, macrophage, inflammatory molecules, airway inflammation, NF-¥êB, acute lung injury
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